An alternating flow-direction method for increasing productivity in the purification of large biotherapeutic modalities using size exclusion chromatography

The purification of large biotherapeutic modalities such as viral coat proteins, plasmid DNA, mRNA, therapeutic viruses and vesicles is more challenging than the purification of smaller and more established products such as monoclonal antibodies. This is because these entities, due to their large size, have limited access to binding sites present in the pores of conventional resin-based chromatographic media. However, this transport limitation …