Journal article
Transition-State Structure for the ADP-Ribosylation of Recombinant Gi α 1 Subunits by Pertussis Toxin †
Abstract
Pertussis toxin ADP-ribosylates a specific Cys side chain in the alpha-subunit of several G-proteins. Recombinant Gialpha1-subunits were rapidly ADP-ribosylated in the absence of betagamma-subunits, with a Km of 800 microM and a kcat of 40 min-1. Addition of betagamma-subunits decreases Km to 0.3 microM with little change of kcat. Kinetic isotope effects established the transition-state structure for ADP-ribosylation of Gialpha1 subunits. The …
Authors
Scheuring J; Berti PJ; Schramm VL
Journal
Biochemistry, Vol. 37, No. 9, pp. 2748–2758
Publisher
American Chemical Society (ACS)
Publication Date
March 1, 1998
DOI
10.1021/bi972594x
ISSN
0006-2960