Journal article
Plant-Produced Trastuzumab Inhibits the Growth of HER2 Positive Cancer Cells
Abstract
To study the agricultural production of biosimilar antibodies, trastuzumab (Herceptin) was expressed in Nicotiana benthamiana using the magnICON viral-based transient expression system. Immunoblot analyses of crude plant extracts revealed that trastuzumab accumulates within plants mostly in the fully assembled tetrameric form. Purification of trastuzumab from N. benthamiana was achieved using a scheme that combined ammonium sulfate …
Authors
Grohs BM; Niu Y; Veldhuis LJ; Trabelsi S; Garabagi F; Hassell JA; McLean MD; Hall JC
Journal
Journal of Agricultural and Food Chemistry, Vol. 58, No. 18, pp. 10056–10063
Publisher
American Chemical Society (ACS)
Publication Date
September 22, 2010
DOI
10.1021/jf102284f
ISSN
0021-8561