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Satellite cell number and cell cycle kinetics in...
Journal article

Satellite cell number and cell cycle kinetics in response to acute myotrauma in humans: immunohistochemistry versus flow cytometry

Abstract

In humans, muscle satellite cell (SC) enumeration is an important measurement used to determine the myogenic response to various stimuli. To date, the standard practice for enumeration is immunohistochemistry (IHC) using antibodies against common SC markers (Pax7, NCAM). Flow cytometry (FC) analysis may provide a more rapid and quantitative determination of changes in the SC pool with potential for additional analysis not easily achievable with …

Authors

McKay BR; Toth KG; Tarnopolsky MA; Parise G

Journal

The Journal of Physiology, Vol. 588, No. 17, pp. 3307–3320

Publisher

Wiley

Publication Date

September 1, 2010

DOI

10.1113/jphysiol.2010.190876

ISSN

0022-3751