Journal article
Single-step analysis of low abundance phosphoamino acids via on-line sample preconcentration with chemical derivatization by capillary electrophoresis
Abstract
New strategies for rapid, sensitive and high-throughput analysis of low abundance metabolites in biological samples are required for future metabolomic research. In this report, a direct method for sub-micromolar analyses of phosphoamino acids was developed using on-line sample preconcentration with 9-fluorenylmethyloxycarbonyl chloride (FMOC) derivatization by capillary electrophoresis (CE) and UV detection. Analyte focusing by dynamic pH …
Authors
Ptolemy AS; Britz-McKibbin P
Journal
Analyst, Vol. 130, No. 9, pp. 1263–1270
Publisher
Royal Society of Chemistry (RSC)
Publication Date
2005
DOI
10.1039/b504480d
ISSN
0003-2654