Journal article
Characterization of vitellogenin gene expression in round goby (Neogobius melanostomus) using a quantitative polymerase chain reaction assay
Abstract
A growing concern over endocrine disruption in aquatic species has prompted the development of molecular assays to monitor environmental impacts. This study describes the development of quantitative polymerase chain reaction (qPCR) assays to characterize the expression of two vitellogenin (Vtg) genes in the invasive round goby (Neogobius melanostomus). Fragments from the 18SrRNA (housekeeping gene), Vtg II, and Vtg III genes were cloned and …
Authors
Bowley LA; Alam F; Marentette JR; Balshine S; Wilson JY
Journal
Environmental Toxicology and Chemistry, Vol. 29, No. 12, pp. 2751–2760
Publisher
Oxford University Press (OUP)
Publication Date
December 1, 2010
DOI
10.1002/etc.324
ISSN
0730-7268