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Properties of NAD+- and NADP+-dependent malic...
Journal article

Properties of NAD+- and NADP+-dependent malic enzymes of Rhizobium (Sinorhizobium) meliloti and differential expression of their genes in nitrogen-fixing bacteroids

Abstract

The wild-type NAD(+)-dependent malic enzyme (dme) gene of Rhizobium (now Sinorhizobium) meliloti was cloned and localized to a 3.1 kb region isolated on the cosmid pTH69. This cosmid complemented the symbiotic nitrogen fixation (Fix-) phenotype of R. meliloti dme mutants. The dme gene was mapped by conjugation to between the cys-11 and leu-53 markers on the R. meliloti chromosome. beta-Galactosidase activities measured in bacterial strains …

Authors

Driscoll BT; Finan TM

Journal

Microbiology, Vol. 143, No. 2, pp. 489–498

Publisher

Microbiology Society

Publication Date

February 1, 1997

DOI

10.1099/00221287-143-2-489

ISSN

1350-0872