A bioorthogonal methylene blue derived probe for targeted photoacoustic imaging

Photoacoustic (PA) imaging is a nonradioactive and noninvasive imaging modality that can volumetrically map the distribution of photo-absorbing molecules with deeper tissue penetration than optical modalities. Here, we report on a convenient strategy to synthesize a modular methylene blue tetrazine (MB-Tz) PA imaging agent and a trans-cyclooctene (TCO)-labeled bisphosphonate (TCO-BP) calcium metabolism targeting vector. PA imaging of MB-Tz …