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In vitro evaluation of CAR-T cells in...
Journal article

In vitro evaluation of CAR-T cells in patient-derived glioblastoma models

Abstract

Advances in chimeric antigen receptor (CAR) T cell therapies have led to the modality dominating translational cancer research; however, a standardized protocol for evaluating such therapies in vitro is needed. This protocol details the in vitro preclinical evaluation of CAR-T cell therapies for glioblastoma (GBM), including target cell cytotoxicity and T cell proliferation, activation, and cytokine release assays. For complete details on the …

Authors

Brakel BA; Chokshi CR; Salim SK; Venugopal C; Singh S

Journal

STAR Protocols, Vol. 2, No. 4,

Publisher

Elsevier

Publication Date

December 2021

DOI

10.1016/j.xpro.2021.100920

ISSN

2666-1667