Journal article
Amperometric biosensor with HRP immobilized on a sandwiched nano-Au / polymerized m-phenylenediamine film and ferrocene mediator
Abstract
Abstract.An amperometric biosensor has been developed for the determination of H2O2 in plant samples. Horseradish peroxidase (HRP) is immobilized on a sandwiched nano-Au particle / m-phenylenediamine polymer film by glutaraldehyde cross-linking. The film is formulated on the carbon paste electrode (CPE) blended with ferrocene as an electron transfer mediator. On the low concentration range, the current response is related to the H2O2 …
Authors
Li J; Xiao L-T; Liu X-M; Zeng G-M; Huang G-H; Shen G-L; Yu R-Q
Journal
Analytical and Bioanalytical Chemistry, Vol. 376, No. 6, pp. 902–907
Publisher
Springer Nature
Publication Date
7 2003
DOI
10.1007/s00216-003-1989-8
ISSN
1618-2642