Journal article
Development and Characterization of a Xylose-Dependent System for Expression of Cloned Genes inBacillus subtilis: Conditional Complementation of a Teichoic Acid Mutant
Abstract
We have developed a xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis. The expression system is contained on plasmid pSWEET for integration at the amyE locus of B. subtilis and incorporates components of the well-characterized, divergently transcribed xylose utilization operon. The system contains the xylose repressor encoded by xylR, the promoter and 5' portion of xylA containing an …
Authors
Bhavsar AP; Zhao X; Brown ED
Journal
Applied and Environmental Microbiology, Vol. 67, No. 1, pp. 403–410
Publisher
American Society for Microbiology
Publication Date
1 2001
DOI
10.1128/aem.67.1.403-410.2001
ISSN
0099-2240