Journal article
Bacterial Binding, Phagocytosis, and Killing: Measurements Using Colony Forming Units
Abstract
Herein we provide a colony forming unit (CFU)-based counting method for quantitating the bacterial binding, phagocytosis, and killing capacity of phagocytes. Although these functions can be measured by immunofluorescence and dye-based assays, quantitating CFUs is comparatively inexpensive and easy to perform. The protocol described below is easily modified for use with different phagocytes (e.g., macrophages, neutrophils, cell lines), types of …
Authors
Novakowski KE; Loukov D; Chawla V; Bowdish DME
Journal
Methods in Molecular Biology, Vol. 1519, , pp. 297–309
Publisher
Springer Nature
Publication Date
2017
DOI
10.1007/978-1-4939-6581-6_20
ISSN
1064-3745