Journal article
An Enhanced Mass Spectrometry Approach Reveals Human Embryonic Stem Cell Growth Factors in Culture
Abstract
The derivation and long-term maintenance of human embryonic stem cells (hESCs) has been established in culture formats that are both dependent and independent of support (feeder) cells. However, the factors responsible for preserving the viability of hESCs in a nascent state remain unknown. We describe a mass spectrometry-based method for probing the secretome of the hESC culture microenvironment to identify potential regulating protein factors …
Authors
Bendall SC; Hughes C; Campbell JL; Stewart MH; Pittock P; Liu S; Bonneil E; Thibault P; Bhatia M; Lajoie GA
Journal
Molecular & Cellular Proteomics, Vol. 8, No. 3, pp. 421–432
Publisher
Elsevier
Publication Date
March 2009
DOI
10.1074/mcp.m800190-mcp200
ISSN
1535-9476