Journal article
CRISPRi screens reveal a DNA methylation-mediated 3D genome dependent causal mechanism in prostate cancer
Abstract
Prostate cancer (PCa) risk-associated SNPs are enriched in noncoding cis-regulatory elements (rCREs), yet their modi operandi and clinical impact remain elusive. Here, we perform CRISPRi screens of 260 rCREs in PCa cell lines. We find that rCREs harboring high risk SNPs are more essential for cell proliferation and H3K27ac occupancy is a strong indicator of essentiality. We also show that cell-line-specific essential rCREs are enriched in the …
Authors
Ahmed M; Soares F; Xia J-H; Yang Y; Li J; Guo H; Su P; Tian Y; Lee HJ; Wang M
Journal
Nature Communications, Vol. 12, No. 1,
Publisher
Springer Nature
DOI
10.1038/s41467-021-21867-0
ISSN
2041-1723
Fields of Research (FoR)
Medical Subject Headings (MeSH)
AnimalsCCCTC-Binding FactorCRISPR-Cas SystemsCarcinogenesisCell Line, TumorDNA MethylationGene EditingGenetic Predisposition to DiseaseGenome-Wide Association StudyHumansMaleMice, Inbred NODMice, SCIDPolymorphism, Single NucleotidePromoter Regions, GeneticProstatic NeoplasmsProto-Oncogene Proteins c-mycQuantitative Trait LociRegulatory Elements, TranscriptionalRisk FactorsMice