Investigation of lysine: N-hydroxylation in the biosynthesis of the siderophore aerobactin

N-Ethylmaleimide was found to inhibit both pyruvate oxidation and lysine: N6-hydroxylation catalyzed by an enzyme system derived from Aerobacter aerogenes 62-1. Studies of pyruvate utilization in the presence and absence of lysine indicated a 1:1 stoichiometric relationship between consumption of pyruvate and production of N6-hydroxylation. Similar studies with an Escherichia coli mutant enzyme system revealed a 2:1 stoichiometric relationship …