Journal article
Evaluation of air-interfaced Calu-3 cell layers for investigation of inhaled drug interactions with organic cation transporters in vitro
Abstract
A physiologically pertinent in vitro model is urgently needed for probing interactions between inhaled drugs and the organic cation transporters (OCT) in the bronchial epithelium. This study evaluated OCT expression, functionality, inhibition by common inhaled drugs and impact on formoterol transepithelial transport in layers of human bronchial epithelial Calu-3 cells grown at an air-liquid interface. 21 day old Calu-3 layers expressed OCT1, …
Authors
Mukherjee M; Pritchard DI; Bosquillon C
Journal
International Journal of Pharmaceutics, Vol. 426, No. 1-2, pp. 7–14
Publisher
Elsevier
Publication Date
4 2012
DOI
10.1016/j.ijpharm.2011.12.036
ISSN
0378-5173
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Administration, InhalationAlbuterolBiological TransportBronchodilator AgentsBudesonideCell LineDose-Response Relationship, DrugEpithelial CellsEthanolaminesFluorescent DyesFormoterol FumarateGene Expression RegulationHumansIpratropiumOrganic Cation Transport ProteinsOrganic Cation Transporter 1Organic Cation Transporter 2PermeabilityPyridinium CompoundsRespiratory MucosaSolute Carrier Family 22 Member 5Symporters