A z2 laterally-fed membrane chromatography device for fast high-resolution purification of biopharmaceuticals

We examine the use of a z² flow distribution and collection feature for enhancing the separation efficiency of a laterally-fed membrane chromatography (or LFMC) device. The new device is designated z²LFMC. Two devices were fabricated using two different ion-exchange membranes: strong anion exchange (Q) with 0.8-micron pore size, and strong cation exchange (S) with pore size in the 3-5 µm range. The number of theoretical plates per unit membrane …