Journal article
An NMR based phosphodiesterase assay
Abstract
We propose a phosphodiesterase assay based on 1D 1H NMR to monitor the hydrolysis of cyclic nucleotides directly, without requiring tags or the addition of exogenous reagents. The method is suitable to measure phosphodiesterase KM and kcat parameters and to identify phosphodiesterase inhibitors.
Authors
Akimoto M; Yu T; Moleschi K; Van K; Anand GS; Melacini G
Journal
Chemical Communications, Vol. 56, No. 58, pp. 8091–8094
Publisher
Royal Society of Chemistry (RSC)
Publication Date
July 25, 2020
DOI
10.1039/d0cc01673j
ISSN
1359-7345