Journal article
Multicopy Suppressors for Novel Antibacterial Compounds Reveal Targets and Drug Efflux Susceptibility
Abstract
Gene dosage has frequently been exploited to select for genetic interactions between a particular mutant and clones from a random genomic library at high copy. We report here the first use of multicopy suppression as a forward genetic method to determine cellular targets and potential resistance mechanisms for novel antibacterial compounds identified through high-throughput screening. A screen of 8640 small molecules for growth inhibition of a …
Authors
Li X; Zolli-Juran M; Cechetto JD; Daigle DM; Wright GD; Brown ED
Journal
Cell Chemical Biology, Vol. 11, No. 10, pp. 1423–1430
Publisher
Elsevier
Publication Date
10 2004
DOI
10.1016/j.chembiol.2004.08.014
ISSN
2451-9456