Microfluidic device for microinjection of caenorhab-ditis elegans

Caenorhabditis elegans is a well-established model organism for neurobiological and drug discovery studies [1]. Microinjection in C.elegans which is crucial for generation transgenic worms, requires expensive multiple degree of freedom (DOF) manipulators, detailed injector alignment procedures and skilled operator which makes the injection process slow and not suitable for scaling to high-throughput. This paper describes the first microinjection device capable of simultaneously immobilizing the worm and inject reagents in a simple one degree of freedom motion that can be easily scaled to high throughput.