Selective functionalization of cantilever biosensors using a microarray spotter

We report a novel method to selectively functionalize small groups of resonant NEMS cantilever based biosensors in an array of devices. This work presents a solution to the long standing problem of device functionalization where it is desirable to treat different subsets of devices on a single chip or array with different compounds. Using a microarray spotter, small volumes (spots) of an oligonucleotide solution were dispensed onto released devices with the intent to hybridize these small DNA strands and detect the additional bound mass through resonant frequency shift. Preliminary data suggests that additional mass selectively bound to the spotted devices. Copyright © 2005 by the Transducer Research Foundation, Inc.