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Ultrasensitive detection of oncogenic human...
Journal article

Ultrasensitive detection of oncogenic human papillomavirus in oropharyngeal tissue swabs

Abstract

BackgroundThe incidence of oropharyngeal squamous cell carcinoma (OPSCC) caused by oncogenic human papillomavirus (HPV) is rising worldwide. HPV-OPSCC is commonly diagnosed by RT-qPCR of HPV E6 and E7 oncoproteins or by p16 immunohistochemistry (IHC). Droplet digital PCR (ddPCR) has been recently reported as an ultra-sensitive and highly precise method of nucleic acid quantification for biomarker analysis. To validate the use of a minimally …

Authors

Isaac A; Kostiuk M; Zhang H; Lindsay C; Makki F; O’Connell DA; Harris JR; Cote DWJ; Seikaly H; Biron VL

Journal

Journal of Otolaryngology, Vol. 46, No. 1,

Publisher

SAGE Publications

Publication Date

January 2017

DOI

10.1186/s40463-016-0177-8

ISSN

1916-0208