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Sperm swim-up techniques and DNA fragmentation
Journal article

Sperm swim-up techniques and DNA fragmentation

Abstract

BACKGROUND: Swim-up techniques for sperm separation may have detrimental effects on sperm DNA. We wished to determine whether the normal swim-up method with centrifugation used in our laboratory, which involves a centrifugation step, was harmful to sperm compared with swim-up without centrifugation. METHODS: Semen samples were obtained from patients undergoing IVF or andrology assessment. An aliquot was removed for fixation and subsequent DNA …

Authors

Younglai EV; Holt D; Brown P; Jurisicova A; Casper RF

Journal

Human Reproduction, Vol. 16, No. 9, pp. 1950–1953

Publisher

Oxford University Press (OUP)

Publication Date

September 1, 2001

DOI

10.1093/humrep/16.9.1950

ISSN

0268-1161