Journal article
LiveFly: A Toolbox for the Analysis of Transcription Dynamics in Live Drosophila Embryos
Abstract
We present the LiveFly toolbox for quantitative analysis of transcription dynamics in live Drosophila embryos. The toolbox allows users to process two-color 3D confocal movies acquired using nuclei-labeling and the fluorescent RNA-tagging system described in the previous chapter and export the nuclei’s position as a function of time, their lineages and the intensity traces of the active loci. The toolbox, which is tailored for the context of …
Authors
Tran H; Perez-Romero CA; Ferraro T; Fradin C; Dostatni N; Coppey M; Walczak AM
Journal
Methods in Molecular Biology, Vol. 1863, , pp. 183–195
Publisher
Springer Nature
Publication Date
2018
DOI
10.1007/978-1-4939-8772-6_11
ISSN
1064-3745