Journal article
Structural model of a synthetic Ca2+ channel with bound Ca2+ ions and dihydropyridine ligand
Abstract
Grove et al. have demonstrated L-type Ca2+ channel activity of a synthetic channel peptide (SCP) composed of four helices (sequence: DPWNVFDFLI10VIGSIIDVIL20SE) tethered by their C-termini to a nanopeptide template. We sought to obtain the optimal conformations of SCP and locate the binding sites for Ca2+ and for the dihydropyridine ligand nifedipine. Eight Ca2+ ions were added to neutralize the 16 acidic residues in the helices. Eight patterns …
Authors
Zhorov BS; Ananthanarayanan VS
Journal
Biophysical Journal, Vol. 70, No. 1, pp. 22–37
Publisher
Elsevier
Publication Date
1 1996
DOI
10.1016/s0006-3495(96)79561-9
ISSN
0006-3495
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl esterAmino Acid SequenceBinding SitesBiophysical PhenomenaBiophysicsCalciumCalcium Channel BlockersCalcium ChannelsDihydropyridinesIn Vitro TechniquesIon Channel GatingLigandsModels, MolecularMolecular Sequence DataMolecular StructureNifedipineProtein ConformationProtein Structure, SecondaryThermodynamics