In Vitro Selection of Structure‐Switching Signaling Aptamers

Picking out probes: A novel approach permits isolation of standard DNA aptamers by in vitro selection and conversion into fluorescence signaling probes. This method comprises the isolation of DNA aptamers capable of duplex‐to‐complex structure switching and labeling the derived aptamers with a pair of short DNA strands with a fluorophore F and a quencher Q to create a reporter system for real‐time sensing (see picture).