Journal article
Capping DNA with DNA †
Abstract
Twelve classes of deoxyribozymes that promote an ATP-dependent "self-capping" reaction were isolated by in vitro selection from a random-sequence pool of DNA. Each deoxyribozyme catalyzes the transfer of the AMP moiety of ATP to its 5'-terminal phosphate group, thereby forming a 5',5'-pyrophosphate linkage. An identical DNA adenylate structure is generated by the T4 DNA ligase during enzymatic DNA ligation. A 41-nucleotide class 1 deoxyribozyme …
Authors
Li Y; Liu Y; Breaker RR
Journal
Biochemistry, Vol. 39, No. 11, pp. 3106–3114
Publisher
American Chemical Society (ACS)
Publication Date
March 1, 2000
DOI
10.1021/bi992710r
ISSN
0006-2960