Journal article
Endogenous peroxidases in sputum interfere with horse-radish peroxidase-based ELISAs
Abstract
Peroxidase-based immunoassays are commonly used for detecting inflammatory mediators in biological samples. We suggest caution while interpreting assays particularly in sputum samples that have endogenous peroxidases like eosinophil peroxidase (EPX), which may interact with a horseradish peroxidase (HRP)-based ELISA. Using IL-8 as an example, we demonstrate that values generated with an HRP-ELISA (n=47) show significant positive correlation …
Authors
Kim LH-Y; Plaza K; Thomas SR; Draijer C; Radford K; Peters-Golden M; Mukherjee M; Nair P
Journal
Journal of Immunological Methods, Vol. 454, , pp. 76–79
Publisher
Elsevier
Publication Date
March 2018
DOI
10.1016/j.jim.2017.11.005
ISSN
0022-1759