Screening of antagonists based on induced dissociation of a calmodulin–melittin interaction entrapped in a sol–gel derived matrix

Sol–gel derived materials offer a unique advantage for the development of sensing and screening platforms in that they allow for the entrapment of multiple species within a confined space. In this work, we show that it is possible to entrap an intact protein–peptide interaction, consisting of bovine calmodulin (bCaM) and melittin, into a sol–gel derived silicate material. Fluorescence emission data demonstrate that the entrapped complex behaves …