Degradation of adsorbed fibrinogen by surface‐generated plasmin

An investigation of the physical status of fibrinogen that has been adsorbed to glass and then eluted has been conducted. Exposure of Kabi fibrinogen to glass was carried out using a glass bead column experiment. The fibrinogen was eluted sequentially, first by 1 M Tris and then by SDS. The initially eluted fibrinogen showed considerable degradation (SDS polyacrylamide gel electrophoresis) while the later fractions were less degraded. …