Journal article
Accuracy and Biological Variation of Human Serum Paraoxonase 1 Activity and Polymorphism (Q192R) by Kinetic Enzyme Assay
Abstract
BACKGROUND: Paraoxonase 1 (PON1) phenotype is a better predictor of atherosclerosis risk than are PON1 genetic polymorphisms alone. Larger studies are required to determine the role of PON1 and there is a need for standardized PON1 assays between laboratories.
METHODS: We have adapted 5 enzyme kinetic assays for high-throughput automated analysis of PON1 activity. Using different substrates and reaction conditions, we measured PON1 activity and …
Authors
Browne RW; Koury ST; Marion S; Wilding G; Muti P; Trevisan M
Journal
Clinical Chemistry, Vol. 53, No. 2, pp. 310–317
Publisher
Oxford University Press (OUP)
Publication Date
February 1, 2007
DOI
10.1373/clinchem.2006.074559
ISSN
0009-9147