Journal article
Separation of monoclonal antibody alemtuzumab monomer and dimers using ultrafiltration
Abstract
This article examines the feasibility of using ultrafiltration to separate the monomer of the monoclonal antibody alemtuzumab (Campath or Campath-1H) from a mixture of dimer and higher-order oligomers (collectively called "dimers" here). Using parameter scanning ultrafiltration, we initially assessed the suitability of the following membranes: 100 kDa and 300 kDa polyethersulfone (PES) membranes, and a 100 kDa polyvinylidene fluoride (PVDF) …
Authors
Wan Y; Vasan S; Ghosh R; Hale G; Cui Z
Journal
Biotechnology and Bioengineering, Vol. 90, No. 4, pp. 422–432
Publisher
Wiley
Publication Date
May 20, 2005
DOI
10.1002/bit.20416
ISSN
0006-3592