Journal article
Purification of humanized monoclonal antibodies by membrane-based hybrid bioseparation technique
Abstract
We describe a rapid, inexpensive and scalable hybrid bioseparation technique for one-step purification of humanized monoclonal antibodies from mammalian cell culture supernatant. It involves the selective and reversible retention of the monoclonal antibody within a membrane module utilizing a combination of microfiltration of precipitated antibody and simultaneous hydrophobic interaction-based membrane chromatography of soluble antibody on the …
Authors
Wang L; Kanani DM; Ghosh R
Journal
Journal of Immunological Methods, Vol. 314, No. 1-2, pp. 1–8
Publisher
Elsevier
Publication Date
July 2006
DOI
10.1016/j.jim.2006.05.002
ISSN
0022-1759