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Ultrafast Separation and Analysis of Monoclonal...
Journal article

Ultrafast Separation and Analysis of Monoclonal Antibody Aggregates Using Membrane Chromatography

Abstract

We discuss a method for rapid and cost-effective analysis of monoclonal antibody (mAb) aggregates. Hydrophobic interaction membrane chromatography, which was previously shown to be highly suitable for such separation and analysis, was used in a recently developed format referred to as laterally fed membrane chromatography (or LFMC). A stack of rectangular polyvinylidene fluoride (or PVDF) membranes having 0.22 μm pores housed within a modified …

Authors

Madadkar P; Umatheva U; Hale G; Durocher Y; Ghosh R

Journal

Analytical Chemistry, Vol. 89, No. 8, pp. 4716–4720

Publisher

American Chemical Society (ACS)

Publication Date

April 18, 2017

DOI

10.1021/acs.analchem.7b00580

ISSN

0003-2700