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Photoaffinity labeling and fatty acid permeation...
Journal article

Photoaffinity labeling and fatty acid permeation in 3T3-L1 adipocytes.

Abstract

Long chain fatty acid uptake was investigated in 3T3-L1 cells. Differentiation of these cells from fibroblasts to adipocytes was accompanied by an 8.5-fold increase in the rate of oleate uptake. This was saturable in adipocytes with apparent Kt and Vmax values of 78 nM and 16 nmol/min/mg cell protein, respectively. A number of proteins in various subcellular fractions of differentiated cells were labeled with the photoreactive fatty acid …

Authors

Trigatti BL; Mangroo D; Gerber GE

Journal

Journal of Biological Chemistry, Vol. 266, No. 33, pp. 22621–22625

Publisher

Elsevier

Publication Date

November 1991

DOI

10.1016/s0021-9258(18)54616-0

ISSN

0021-9258