Journal article
Photoaffinity labeling and fatty acid permeation in 3T3-L1 adipocytes.
Abstract
Long chain fatty acid uptake was investigated in 3T3-L1 cells. Differentiation of these cells from fibroblasts to adipocytes was accompanied by an 8.5-fold increase in the rate of oleate uptake. This was saturable in adipocytes with apparent Kt and Vmax values of 78 nM and 16 nmol/min/mg cell protein, respectively. A number of proteins in various subcellular fractions of differentiated cells were labeled with the photoreactive fatty acid …
Authors
Trigatti BL; Mangroo D; Gerber GE
Journal
Journal of Biological Chemistry, Vol. 266, No. 33, pp. 22621–22625
Publisher
Elsevier
Publication Date
November 1991
DOI
10.1016/s0021-9258(18)54616-0
ISSN
0021-9258