Effect of gamma-thio-ATP on dog stomach smooth muscle membranes.
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The effect of gamma-Thio-ATP on high affinity ATPase and on the ATP-dependent Ca-uptake of a plasma membrane fraction prepared from the dog stomach smooth muscle was examined. gamma-Thio-ATP did not act as a substrate for the ATP-dependent Ca-uptake and was only a poor substrate for the high affinity Ca-ATPase reaction. The compound, however, reversibly inhibited the uptake and the ATPase.
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