Journal article
Nerve growth factor treatment alters Ca2+ pump levels in PC12 cells
Abstract
Nerve growth factor (NGF) treatment converts rapidly dividing PC12 cells into a neuronal phenotype. To understand the Ca2+ sequestration mechanisms accompanying this differentiation, we examined the endoplasmic reticulum Ca2+ (SERCA) pump levels using two different assays: ATP-dependent azide insensitive oxalate stimulated 45Ca2+ uptake by PC12 cells permeabilized with saponin, and Western blots using a monoclonal antibody which reacts with all …
Authors
Keller D; Grover AK
Journal
Neuroreport, Vol. 11, No. 1, pp. 65–68
Publisher
Wolters Kluwer
Publication Date
January 2000
DOI
10.1097/00001756-200001170-00013
ISSN
0959-4965