Endothelium and smooth muscle of pig coronary artery: differences in metabolism.
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Here, we report that the smooth muscle and endothelium of the pig coronary artery differ in the profiles of energy metabolism nucleotides. ATP levels in the freshly isolated smooth muscle (1490 +/- 93, all the values are in pmol/mg protein) were significantly greater than in the endothelium (418 +/- 68). In contrast, endothelium contained higher levels of NADH (328 +/- 21), NAD+ (1210 -/+ 28), NADPH (87 +/- 2), and NADP+ (77 +/- 4) than smooth muscle (17 +/- 2, 96 +/- 14, 7 +/- 1, and 8 +/- 1, respectively). However, smooth muscle and endothelium do not differ from each other in the ratios of NADH/NAD+ or NADPH/NADP+. Cells cultured from smooth muscle and endothelium contained less ATP (93 +/- 2, 141 +/- 6) and had lower ratios of NADH/ NAD+ than the freshly isolated tissues but the NADPH/NADP+ ratios remained similar. We conclude that (a) freshly isolated smooth muscle and endothelium differ in their profiles of the energy metabolism nucleotides, and (b) culturing the cells alters the profile.
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