INDUCTION OF ANTIIDIOTYPIC ANTIBODIES BY BLOOD TRANSFUSIONS Journal Articles

abstract

• Balb/c (H-2d) mice were transfused weekly with 0.1 ml of whole blood from C3H (H-2k) mice. One week after 3 blood transfusions (BT), the mice were bled and the sera collected and pooled. The 3BT serum was absorbed twice with C3H lymphocytes and IgG isolated by ion-exchange chromatography. Balb/c anti-C3H, Balb/c anti-Balb/c, and Balb/c anti-SJL (H-2s) lymphocytes were generated in the mixed lymphocyte cultures and metabolically labeled with 35S-methionine. Cell lysates were prepared from labeled lymphocytes and precleared by absorption with normal mouse serum. Immunoprecipitation was carried out by 3BT-IgG and NMS-IgG. 3BT-IgG specifically precipitated 7 molecules (30K, 60K, 72K, 86K, 92K, 97K, 145K) from Balb/c anti-C3H lymphocytes. In contrast, 3BT-IgG did not precipitate these molecules from Balb/c anti-Balb/c or from Balb/c anti-SJL lymphocytes. The data suggest that BT induces antibodies directed against the blood donor alloantigen-specific receptors on recipient's T lymphocytes.

authors

• SINGAL, DP
• LUDWIN, D
• JOSEPH, S
• BLAJCHMAN, MA
• Ofosu, Frederick A
• LIAO, S-K

status

• published 

publication date

• December 1986

has subject area

• 11 Medical and Health Sciences (FoR)
• Animals (MeSH)
• Antigens, Surface (MeSH)
• Blood Transfusion (MeSH)
• Immunoglobulin Idiotypes (MeSH)
• Mice (MeSH)
• Mice, Inbred BALB C (MeSH)
• Mice, Inbred C3H (MeSH)
• Molecular Weight (MeSH)
• Receptors, Antigen, T-Cell (MeSH)
• Surgery (Science Metrix)
• T-Lymphocytes (MeSH)

published in

• Transplantation  Journal

keywords

• Animals
• Antigens, Surface
• Blood Transfusion
• Immunoglobulin Idiotypes
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C3H
• Molecular Weight
• Receptors, Antigen, T-Cell
• T-Lymphocytes

Digital Object Identifier (DOI)

• 10.1097/00007890-198612000-00011

PubMed ID

• 3491450

start page

• 632

end page

• 635

volume

• 42

issue

• 6