Journal article
A Sol−Gel-Derived Acetylcholinesterase Microarray for Nanovolume Small-Molecule Screening
Abstract
A fluorimetric acetylcholinesterase (AChE) assay was developed and characterized both in solution and with the enzyme entrapped in sol-gel-derived silica. The assay is based on a disulfide-thiol interchange reaction between the intramolecularly quenched dimeric dye BODIPY FL l-cystine and thiocholine generated by the AChE-catalyzed hydrolysis of acetylthiocholine (ATCh), which results in a brightly fluorescent monomeric product owing to the …
Authors
Monton MRN; Lebert JM; Little JRL; Nair JJ; McNulty J; Brennan JD
Journal
Analytical Chemistry, Vol. 82, No. 22, pp. 9365–9373
Publisher
American Chemical Society (ACS)
Publication Date
November 15, 2010
DOI
10.1021/ac101949s
ISSN
0003-2700