Construction of a recombinant adenovirus for efficient delivery of the I-Scel yeast endonuclease to human cells and its application in the in vivo cleavage of chromosomes to expose new potential telomeres

We have constructed a replication-defective adenovirus vector encoding the yeast I- Sce I endonuclease under the control of the murine cytomegalovirus immediate-early gene promoter (AdM Sce I) for efficient delivery of this enzyme to mammalian cells. We present evidence of AdM Sce I-mediated I- Sce I protein expression and cleavage activity in replication-permissive 293 cells, and of cleavage of chromosomes in vivo in both 293 cells and in …