The use of fluorogenic substrates to locate rapidly enzyme activity in chromatographic fractions

A rapid method of screening chromatographic fractions has been developed for enzymes which metabolize fluorogenic substrates. Samples of the eluted fractions are applied to cellulose acetate gels and then incubated with the specific fluorogenic substrate. Fractions which possess enzymatic activity are visible as fluorescent spots when the gels are examined under long-wave ultraviolet light.