Radioimmunoassay for Dog Myoglobin

A radioimmunoassay specific for dog myoglobin (MGB) has been developed. Antibody (Ab) to purified dog heart MGB was prepared in rabbits and the Ab was labelled with 125I using the chloramine-T procedure. The assay is conducted at room temperature with addition of second Ab at 24 hr, the forward reaction being 95% complete at this point and irreversible. The assay is free of matrix affects. The working range is 20 to 360 ng/ml. Coefficients of variation for within run replicates are 10.5% (low) and 4.4% (high), and for between run replicates are 13.8% (low) and 7.6% (high). Serial measurement of serum MGB in dogs undergoing experimental myocardial infarction indicates that appearance time, peak time, and disappearance rate are respectively, 1.1 hr, 7.7 hr and 0.0025 min-1 for MGB and 2.6 hr, 13.35 hr, and 0.0018 min-1 for CK.