Journal article
Measurement of Interleukin 6
Abstract
Interleukin 6 (IL-6) is a pluripotent cytokine with multiple effects on many different cell types. It is produced by a variety of cells in response to immunological and other stimuli. This unit describes a simple and sensitive assay for human, rat, rabbit, pig, and mouse IL-6, based on IL-6-dependent proliferation of a murine B cell hybridoma cell line, B9. Support protocols discuss maintenance of B9 cells, preparation of IL-6 standards, and …
Authors
Nordan RP; Richards CD; Gauldie J
Journal
Current Protocols in Immunology, Vol. 17, No. 1, pp. 6.6.1–6.6.5
Publisher
Wiley
Publication Date
March 1996
DOI
10.1002/0471142735.im0606s17
ISSN
1934-3671