Journal article
Optimal conditions for immunohistochemical determination of the in vitro DNA synthesis labelling index with bromodeoxyuridine in head and neck cancer
Abstract
The in vitro DNA synthesis labelling index was assessed immunohistochemically in 24 freshly obtained specimens of head and neck cancer using bromodeoxyuridine (BrdUrd) as the DNA precursor to determine the influence of BrdUrd concentration on labelling index (LI). Initially, tumour fragments were incubated in varying concentrations of BrdUrd from 2 to 100 microM for 2 h, and BrdUrd was detected with an anti-BrdUrd monoclonal antibody using …
Authors
Browman GP; Kanclerz A; Booker L; Daya D; Archibald SD; Young JEM; Goldsmith CH
Journal
Cell Proliferation, Vol. 24, No. 6, pp. 579–585
Publisher
Wiley
Publication Date
11 1991
DOI
10.1111/j.1365-2184.1991.tb01186.x
ISSN
0960-7722