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Fate of Thrombin and Thrombin-Antithrombin-III...
Journal article

Fate of Thrombin and Thrombin-Antithrombin-III Complex Adsorbed to a Heparinized Biomaterial: Analysis of the Enzyme-Inhibitor Complexes Displaced by Plasma

Abstract

Heparin covalently-linked to polyvinyl alcohol (PVA) is a biomaterial which is of potential value as a non-thrombogenic coating. 125I-labelled thrombin adsorbed to heparin-PVA beads was not dislodged by phosphate-buffered saline, pH 7.4, although radioactivity was progressively displaced from the adsorbent by fibrinogen-free human plasma. Analysis by gel filtration and affinity chromatography showed that the released radioactivity was …

Authors

Hatton MWC; Rollason G; Sefton MV

Journal

Thrombosis and Haemostasis, Vol. 50, No. 04, pp. 873–877

Publisher

Thieme

Publication Date

1983

DOI

10.1055/s-0038-1665332

ISSN

0340-6245