Tutorial review. Future prospects for the analysis of complex biological systems using micro-column liquid chromatography–electrospray tandem mass spectrometry Academic Article uri icon

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abstract

  • An overview is provided of methods for the study of complex biological processes by using micro-column liquid chromatography-electrospray ionization tandem mass spectrometry. Procedures discussed include electrospray ionization, micro-column liquid chromatography, tandem mass spectrometry, tandem mass spectra data interpretation for peptides, and database searching with mass spectral data. Several problems in immunology are discussed to illustrate this approach.

authors

  • Koutny, Maciej
  • Yates, John R
  • McCormack, Ashley L
  • Link, Andrew J
  • Schieltz, David
  • Eng, Jimmy
  • Hays, Lara

publication date

  • July 1996