Journal article
Protein-Doped Monolithic Silica Columns for Capillary Liquid Chromatography Prepared by the Sol−Gel Method: Applications to Frontal Affinity Chromatography
Abstract
The development of bioaffinity chromatography columns that are based on the entrapment of biomolecules within the pores of sol-gel-derived monolithic silica is reported. Monolithic nanoflow columns are formed by mixing the protein-compatible silica precursor diglycerylsilane with a buffered aqueous solution containing poly(ethylene oxide) (PEO, MW 10,000) and the protein of interest and then loading this mixture into a fused-silica capillary …
Authors
Hodgson RJ; Chen Y; Zhang Z; Tleugabulova D; Long H; Zhao X; Organ M; Brook MA; Brennan JD
Journal
Analytical Chemistry, Vol. 76, No. 10, pp. 2780–2790
Publisher
American Chemical Society (ACS)
Publication Date
May 1, 2004
DOI
10.1021/ac0352124
ISSN
0003-2700