Journal article
Capillary-Scale Monolithic Immunoaffinity Columns for Immunoextraction with In-Line Laser-Induced Fluorescence Detection
Abstract
A bimodal meso/macroporous monolithic silica capillary column containing an entrapped antibody was prepared by a biocompatible sol-gel process and used for nanoflow immunoaffinity chromatography and immunoextraction studies. Stationary phases were prepared by combining the protein-compatible silane precursor diglycerylsilane with an aqueous solution containing 10,000 Da poly(ethylene glycol) and the antibody. An analytical method was developed …
Authors
Hodgson RJ; Brook MA; Brennan JD
Journal
Analytical Chemistry, Vol. 77, No. 14, pp. 4404–4412
Publisher
American Chemical Society (ACS)
Publication Date
July 1, 2005
DOI
10.1021/ac048142p
ISSN
0003-2700