Altered expression of sodium pump isoforms in the inflamed intestine of Trichinella spiralis-infected rats

We used polymerase chain reaction (PCR) and Northern analysis to examine the effect of Trichinella spiralis infection on mRNA expression of sodium pump isoforms in rat intestine. 28S RNA was coamplified as an internal standard, and the abundance of the isoformic mRNA was expressed relative to 28S RNA. As assessed by PCR and Northern analysis, the alpha 1- and beta 1-subunits were expressed in the jejunum and ileum of noninfected control rats. …